Figure 1.
Postnatal experimental design. Pregnant Wistar dams were exposed to normoxia (N; 21% O2) or (hypoxia (H; 13%-14% O2) from 6 to 20 days gestation (dGA) ± MitoQ (NM and HM, respectively) provided at 500 μM day−1 in the maternal drinking water. Offspring were born and maintained in normoxia until 4 months of age. One male per litter was randomly selected for either conscious in vivo cardiovascular testing in chronically instrumented preparations or ex vivo analysis of cardiac function using an isolated heart Langendorff preparation. For in vivo testing, offspring were instrumented with femoral vascular catheters and a femoral transonic flow probe placed in the contralateral leg. Basal and stimulated in vivo cardiovascular function was determined following 4-5 days of postsurgical recovery