Table 1. SPR fitting parameters of the interaction of anti-AGT mAb with AGT, a 1 : 1 binding model was applied to fit the data.

AGT binding parameters
	k on (M−1 s−1)	k off (s−1)	K d (nM)
Oxidised AGT	1121 ± 4	(1.78 ± 0.01) × 10−4	159 ± 1
Reduced AGT	1540 ± 6	(1.55 ± 0.01) × 10−4	103.1 ± 0.9
Independent Samples t-test (P-value)	<0.0001	<0.0001	<0.0001
t-value	−107.89	32.53	83.10
Degrees of freedom	5	5	5
Individual measurements, oxidised AGT
Anti-AGT mAb surface	kon (M−1 s−1)	koff (s−1)	Kd (nM)
Surface 1	1182 ± 4	(1.78 ± 0.01) × 10−4	150 ± 1
Surface 2	1153 ± 4	(1.73 ± 0.01) × 10−4	150 ± 1
Surface 3	1055 ± 4	(1.84 ± 0.01) × 10−4	174 ± 1
Surface 4	1093 ± 3	(1.77 ± 0.01) × 10−4	162 ± 1
Individual measurements, reduced AGT
Anti-AGT mAb surface	kon (M−1 s−1)	koff (s−1)	Kd (nM)
Surface 1	1439 ± 6	(1.61 ± 1) × 10−4	112 ± 1
Surface 2	1534 ± 6	(1.49 ± 1) × 10−4	97.3 ± 0.9
Surface 3	1596 ± 6	(1.51 ± 1) × 10−4	94.8 ± 0.9
Surface 4	1473 ± 5	(1.59 ± 1) × 10−4	108.1 ± 0.8