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. Author manuscript; available in PMC: 2022 Jan 3.
Published in final edited form as: Sci Signal. 2019 Aug 6;12(593):eaav9150. doi: 10.1126/scisignal.aav9150

Fig. 1. PKD3 is the predominant PKD isoform expressed and activated in liver.

Fig. 1

A Quantification of the four most abundant DAG classes in livers of normal diet (ND) or high-fat diet (HFD) fed mice determined by LC-MS (n=7 mice/diet).

B, C Western blot analysis of PKD-pSer731/735 in isolated primary hepatocytes stimulated with cell-permeable 1,2-Dioctanoyl-sn-glycerol (DAG) (100 μM) (B) or oleic acid (750 μM) (B) for the indicated time points, respectively (n=3 independent experiments).

D Western blot analysis of the indicated proteins from the livers of ND or HFD fed mice (n=3 mice/diet).

E Absolute quantification of PKD isoform expression in liver by QPCR with in-exon primers and normalizing values to a genomic DNA standard (n=8 biological replicates/group, combined data from three independent experiments).

Data information: In (A, E), data are presented as mean ± SEM. *P>0.05 (unpaired two-tailed Student’s t-test (A) or one way ANOVA with post hoc Tukey’s test (E)).