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. Author manuscript; available in PMC: 2022 Jan 21.
Published in final edited form as: Nature. 2020 Jul 16;584(7822):635–639. doi: 10.1038/s41586-020-2557-5

Figure 1. Definition of the MIWI2 interactome and identification of SPOCD1 from gonocytes undergoing de novo genome methylation.

Figure 1

a, Volcano plot showing enrichment (log2(mean LFQ ratio of HA-MIWI2 IP/control IP from Miwi2+/+ foetal testis) and confidence (-log10(P-value of two-sided Student’s t-test)) of proteins co-purifying with HA-MIWI2 from E16.5 testis lysates (n=3). Dotted line indicates factors with enrichment >4-fold and significance P<0.05. Red: Known piRNA pathway members, blue: SPOCD1. b, List of known piRNA biogenesis factors and non-piRNA pathway-associated proteins co-purifying with HA-MIWI2. Novel identified MIWI2 interactors are underlined. c, Relative expression of indicated transcripts as measured by Affymetrix microarray in E16.5 gonocytes (n=2), adult spermatogonia (n=3) and spermatocytes (n=3). Data are mean and s.e.m., normalized to peak expression of each transcript. d, Schematic representation of SPOCD1 domain structure.