Extended Data Table 2. IP-MS analysis of pTIR1::TIR1-VENUS and pAFB1::AFB1-VENUS .

Overview of the putative TIR1 and AFB1 interactors after MaxQuant and Perseus statistical analysis. Samples were TIR1 and AFB1-Venus lines under mock, 50 μM MG132 pre-incubation for 1 hour and 100 nM IAA treatment for 2 minutes. Proteins passing the threshold of FDR 0.05 and specific fold change are included in the table. P-values are calculated based on the three replicates using a Two-sided t-test. Pulldowns were performed in triplicate. Yellow highlight indicates the respective bait protein.

TIR1 with Mock

LQG2(TIR1/Control)	X-LQG10FDI	Peptides	Student’s T-test	Majority protein IDs	Protein names
5.118258158	3.27648164	17	10.15708944	Q39255	SKP1-like protein 1A
4.754890442	2.34634325	10	5.760780734	Q570C0	Protein TRANSPORT INHIBITOR RESPONSE 1
3.60787646	2.94711492	2	8.340412262	P38666;Q42347	60S ribosomal protein L24-2;60S ribosomal protein L24-1
2.98809878	1.73797235	2	3.851309445	P16180	30S ribosomal protein S17, chloroplastic
2.459372203	0.85221949	2	1.834243789	Q94AH6	Cullin-1
2.273972829	2.46638828	2	6.212296758	F4JZ17;Q39129	Thiosulfate sulfurtransferase 16, chloroplastic
2.201576869	0.96994873	2	2.070539977	Q42431	Oleosin 20.3 kDa
2.001925786	0.87062371	4	1.870823782	F4JVC0;F4JVC1;Q03251;F4JV	Glycine-rich RNA-binding protein 8
1.988611857	1.64404399	15	3.603054736	Q9FHW7	SKP1-like protein 1B
1.940104802	1.79712208	3	4.013188676	Q9MAH3	Protein DJ-1 homolog B
1.933815002	0.86164735	2	1.852967808	P59230;Q8VZB9;P59231	60S ribosomal protein L10a-2;60S ribosomal protein L10a-1;60S ribosomal protein L10a-3
1.823474248	1.06487476	2	2.2658229	Q8LA13;Q84W89;Q9M2F9	DEAD-box ATP-dependent RNA helicase 1 1;DEAD-box ATP-dependent RNA helicase 37;DEAD-box ATP-dependent RNA helicase 52
1.708749771	0.93651248	3	2.002836801	P16127;Q5XF33	Magnesium-chelatase subunit ChlI-1, chloroplastic;Magnesium-chelatase subunit ChlI-2, chloroplastic
1.60286204	1.15507001	2	2.456251697	Q9LU14	GDSL esterase/lipase APG