Extended Data Table 3. IP-MS analysis of p35S::TMK1-GFP.
Overview of the putative TMK1 interactors after MaxQuant and Perseus statistical analysis. The list is sorted based on the ratio of 35S::TMK1-GFP vs WT control. Proteins passing the threshold of FDR 0.05 and specific fold change are included in the table. P-values are calculated based on the three replicates using a two-sided t-test. Yellow highlight indicates bait, green indicates GFP and orange indicates selected proteins.
| Average V | Average TM | Ratio | P-value | Fasta.headers |
|---|---|---|---|---|
| 4.68 | 7.67 | 963.19 | 9.27E-05 | Symbols: TMK1 | transmembrane kinase 1 | chr1:24631503-24634415 FORWARD LENG |
| 5.59 | 6.98 | 24.32 | 2.77E-01 | Symbols: | Protein prenylyltransferase superfamily protein | chr5:5679995-5685597 FORW |
| 4.51 | 5.85 | 21.80 | 3.99E-03 | Symbols: PGP10 | P-glycoprotein 10 | chr1:3538470-3543782 REVERSE LENGTH=1227; |
| 4.64 | 5.81 | 14.75 | 3.25E-02 | Symbols: PIP2F, PIP2;4 | plasma membrane intrinsic protein 2;4 | chr5:24375673-2437693 |
| 6.01 | 7.11 | 12.53 | 3.11E-05 | Symbols: AHA1, PMA, OST2, HA1 | H(+)-ATPase 1 | chr2:8221858-8227268 FORWARD |
| 5.06 | 6.10 | 10.99 | 1.34E-01 | ALDOA_RABIT | Fructose-bisphosphate aldolase A (EC 4.1.2.13) (Muscle-type aldolase) |
| 5.84 | 6.87 | 10.73 | 1.74E-03 | Symbols: TMP-C, PIP1;4, PIP1E | plasma membrane intrinsic protein 1;4 | chr4:186569-18 |
| 5.38 | 6.21 | 6.75 | 4.73E-02 | Symbols: PIP2B, PIP2;2 | plasma membrane intrinsic protein 2 | chr2:15613624-15614791 |
| 6.91 | 7.67 | 5.80 | 3.66E-04 | Symbols: | Mannose-binding lectin superfamily protein | chr5:14090825-14092808 FORWA |
| 5.19 | 5.95 | 5.69 | 1.11E-01 | Symbols: MED19A | MED19A;BEST Arabidopsis thaliana protein match is: unknown protei |
| 5.60 | 6.36 | 5.67 | 2.41E-01 | Symbols: | Translation protein SH3-like family protein | chr1:3136407-3137430 REVERSE |
| 5.86 | 6.59 | 5.46 | 2.91E-01 | LYSC_CHICK | Lysozyme C (EC 3.2.1.17) (1,4-beta-N-acetylmuramidase C) (Allergen Gal |
| 5.55 | 6.26 | 5.21 | 2.42E-01 | Symbols: | Translation protein SH3-like family protein | chr3:18504311-18504751 FORWA |
| 5.69 | 6.36 | 4.69 | 3.21E-01 | Symbols: | Ribosomal protein S5 domain 2-like superfamily protein | chr5:6090128-609069 |
| 4.89 | 5.49 | 4.05 | 9.96E-02 | Symbols: PIP2A, PIP2, PIP2;1 | plasma membrane intrinsic protein 2A | chr3:19803906-19 |
| 7.24 | 7.82 | 3.74 | 1.39E-01 | GFP AEQVI | Green fluorescent protein;eGFP from pK7WGF2;eGFP from pK7FWG2;GF |