| pTMK1 |
Fw |
GGGGACAACTTTGTATAGAAAAGTTGCATCGTTTGGAACAGATCTTTCAGC |
AT1G66150 |
for cloning TMK1 promoter |
|
Rv |
GGGGACTGCTTTTTTGTACAAACTTGCAGCTTGAAGAAACAGAGGATTGAAG |
|
|
| gTMK1 |
Fw |
GGGGACAAGTTTGTACAAAAAAGCAGGCTCAATGAAGAAAAGAAGAACCTT |
TAT1G66150 |
for cloning genomic TMK1 |
|
Rv |
GGGGACCACTTTGTACAAGAAAGCTGGGTCTCGTCCATCTACTGAAGTGAAT |
|
|
| cTMK1 |
Fw |
caccATGAAGAAAAGAAGAACCTTTC |
AT1G66150 |
for cloning cDNA of TMK1 |
|
Rv |
TCGTCCATCTACTGAAGTGAATG |
|
|
| cTMK1-K616R |
Fw |
CGAAGATTGCGGTTAGGAGAATGGAGAATGG |
AT1G66150 |
for cloning cDNA of TMK1-K616 |
|
Rv |
CCATTCTCCATTCTCCTAACCGCAATCTTCG |
|
|
| cTMK1-K616E |
Fw |
CGAAGATTGCGGTTGAGAGAATGGAGAATG |
AT1G66150 |
for cloning cDNA of TMK1-K616 |
|
Rv |
CATTCTCCATTCTCTCAACCGCAATCTTCG |
|
|
| TAS_AHA |
Fw |
CCTTTTTCTCCATGACCCTATGCATTTTACCAA |
same gene ID as in qPCR |
for cloning syn-tasiRNA target sequence targeting AHA1/2/7/11 |
|
Rv |
GTTCTTGGTAAAATGCATAGGGTCATGGAGAAAAAGGAGAGTAAGAATGT GAAAAAGTTGCAGGAGAATGTGAAAAAGCTGCAGGAGA |
| qEF1a |
Fw |
TGAGCACGCTCTTCTTGCTTTCA |
AT5G60390 |
for qrtPCR |
|
Rv |
GGTGGTGGCATCCATCTTGTTACA |
|
|
| qAHA1 |
Fw |
GCCAGCTTGTTTGACAACAGGAC |
AT2G18960 |
for qrtPCR |
|
Rv |
TTTGGCTGCAGACCGTGCAATG |
|
|
| qAHA2 |
Fw |
ACATTGACGGCAGTGGTAACTGG |
AT4G30190 |
for qrtPCR |
|
Rv |
TTTGGCAAGTTCGAGGATCTGC |
|
|
| qAHA7 |
Fw |
TGCTCTAAGTGGCAAATCATGGG |
AT3G60330 |
for qrtPCR |
|
Rv |
TTCTTTCCTGTGAGTGCAGTCCTC |
|
|
| qAHA11 |
Fw |
CATTGGCGGTATCCCAATTGCC |
AT5G62670 |
for qrtPCR |
|
Rv |
CCTCTTGGTTATTGCTCCCTGCTG |
|
|
