Figure 2. Overexpression of CDC25A, CCNE1, or MYC leads to upregulation of 52 genes.
(A) RNAseq data of 5 cell lines were corrected for cell line-specific and doxycycline treatment effects on gene expression. Subsequently, PERMANOVA was used to identify common differentially expressed genes upon oncogene expression. 52 genes were found to be commonly upregulated whereas no genes were found to be commonly downregulated in response to induction of CCNE1, CDC25A or MYC (B) Gene Set Enrichment Analysis using two-sided Welch’s t-test for the MSigDB Hallmark collection. An orange box indicates enrichment for upregulated genes due to overexpression of oncogenes in corresponding cell lines, and a green box indicates enrichment for downregulated genes.