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. Author manuscript; available in PMC: 2022 Mar 3.
Published in final edited form as: J Am Chem Soc. 2020 Dec 2;142(50):21102–21109. doi: 10.1021/jacs.0c09681

Figure 2. ATP-fueled transient DSD with transient formation of Output1.

Figure 2

(a) Schematic illustration for coupling ATP-driven ligation/restriction ERNs with DSD. T exp = 37.0 °C, T m of Intermediate1 = 34.0 °C. (b) AGE analyses of DSD with time for different sets of enzyme and ATP concentrations. (c) Fraction of DSD for the systems for different concentrations of BsaI and ATP. Lines are guides to the eye. Most relevant concentrations are indicated within the Figure. Concentration of T4 DNA ligase is 0.8 WU. Concentration of each DNA species is 10 μM.