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. 2022 Mar 8;12(3):712–729. doi: 10.1158/2159-8290.CD-20-0930

Figure 7.

Figure 7. Efficacy of combined dasatinib and trametinib on ex vivo brain slice preparations. A, Coronal slices of normal mouse brain, counterstained with Hoechst 33342 (aqua), are implanted in the pontine region with ICR-B169 parental cells, stained with human nuclear antigen (orange), and treated for 4 days with 1 μmol/L dasatinib, 0.1234 μmol/L trametinib, or both compared with vehicle control. Scale bars, 2 mm. B, Bar plot of quantification of tumor cell infiltration across the brain parenchymal tissue as measured by the calculated area invaded compared with vehicle control. Plotted is the mean of at least six independent slices; error bars represent the SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; ns, not significant, FDR-corrected t test.

Efficacy of combined dasatinib and trametinib on ex vivo brain slice preparations. A, Coronal slices of normal mouse brain, counterstained with Hoechst 33342 (aqua), are implanted in the pontine region with ICR-B169 parental cells, stained with human nuclear antigen (orange), and treated for 4 days with 1 μmol/L dasatinib, 0.1234 μmol/L trametinib, or both compared with vehicle control. Scale bars, 2 mm. B, Bar plot of quantification of tumor cell infiltration across the brain parenchymal tissue as measured by the calculated area invaded compared with vehicle control. Plotted is the mean of at least six independent slices; error bars represent the SD. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; ns, not significant, FDR-corrected t test.