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. Author manuscript; available in PMC: 2022 Mar 29.
Published in final edited form as: Chembiochem. 2019 Oct 1;20(20):2666–2673. doi: 10.1002/cbic.201900167

Figure 2. Operation of the device.

Figure 2

a) Side view (x-z) schematic showing how the control layer, situated above the fluidic layer, can be pressurized to lower the ring-valve or micro-stamps. Top: A single GUV is trapped at the posts. Middle: The ring-valve is actuated. Bottom: The micro-stamp is lowered to compress the GUV. b) The micro-stamp height can be precisely controlled by increasing the applied pressure in the control layer. The height of the micro-stamp above the glass coverslip is measured in front of the posts in the center where GUVs are captured. Note that the center of the stamp is prevented from making contact with the glass due to the presence of the PDMS posts. Averages are from three different chambers and errors bars taken from the standard deviation.