Figure 3. Lactate-sustained lipid metabolism links to histone hyperacetylation in PCa cells.

a) Acetyl-CoA measured in DU145 cells treated with HPF- or CAF-CM and 15mM lactate ± iMCT1. b) Incorporation of ¹⁴C lactate into histones extracted as in Methods, from DU145 cells treated as indicated. c-g) Immunoblot using the indicated antibodies for DU145 cells treated as indicated ± iMCT1, simvastatin, ATGListatin and etomoxir. h-m) Invasion assays performed in DU145 cells with the same conditions and drug treatments as in Fig.2c-g.