Extended Data Fig. 4. Wilbur Anderson Assay.

Wilbur Anderson assay for CA immobilised on the surface of planar ITO, Meso ITO and with the enzyme in solution. Enzyme loadings (in mg) were calculated from QCM studies (Figure S1) for planar surfaces (Planar ITO), from the amount dropcast (Meso ITO due to its high surface area) or the total amount added to solution (solution). Solution conditions: 20 mM Tris buffer, pH 8.3, T= 2 °C