Extended Data Fig. 5. MTR1 as alkyltransferase ribozyme and pH dependence.

(a, b) Chemical structures of substrates (adenosine and benzyl guanine cofactor) before the RNA-catalyzed reaction, and after transfer to the target adenosine in R1 and release of guanine. (c) Overlay of active sites containing m¹A, bn¹A or ab¹A in stick representation. (d) Kinetics of benzyl group transfer at pH 6.0 and pH 7.5, with exemplary gel images as inset. (e) Same as (d), but reaction with ab⁶G. The fastest reaction was observed with ab⁶G at pH 6.0, yielding ca 90% ab¹A-RNA within 2 min. All reactions were performed in duplicate, individual data points and representative gel images are shown (timepoints shown in gels up to 60 min).