Skip to main content
. Author manuscript; available in PMC: 2022 May 12.
Published in final edited form as: Nat Struct Mol Biol. 2021 Oct 4;28(10):811–824. doi: 10.1038/s41594-021-00661-y

Figure 2. Perturbing PRC1 has immediate effects on gene expression.

Figure 2

(A) MA plots depicting gene expression changes in PRC1deg cells treated with IAA for the indicated times relative to untreated cells. Differentially expressed genes (padj < 0.05, fold change > 1.5) are labelled in red.

(B) Distribution of different gene classes among genes with increased expression at different times following IAA treatment. Non-NMI, genes lacking a non-methylated CGI; Non-PcG, non-Polycomb-occupied genes; PcG, Polycomb-occupied genes.

(C) A Venn diagram showing the overlap between significantly derepressed genes identified after treating cells with IAA for the indicated times.

(D) Heatmaps depicting gene expression (cnRNA-seq) in untreated cells and changes following IAA treatment for the three groups of genes defined by the earliest time of derepression. Heatmaps are sorted by RING1B cChIP-seq signal as in (E).

(E) Heatmaps showing cChIP-seq signal for Polycomb chromatin domain features (RING1B, H2AK119ub1, SUZ12, and H3K27me3) in untreated cells, at promoters of the gene groupings in (D). Heatmaps are sorted by RING1B cChIP-seq signal.

(F) As in (E) but for total Pol II and H3K4me3 cChIP-seq.