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. Author manuscript; available in PMC: 2022 May 17.
Published in final edited form as: Science. 2022 May 13;376(6594):eabl5197. doi: 10.1126/science.abl5197

Fig. 2. Myeloid compartment across tissues.

Fig. 2

(A) UMAP visualization of the cell populations in the myeloid compartment. (B) Dot plot for expression of marker genes of the identified myeloid populations. Color represents maximum-normalized mean expression of cells expressing marker genes, and size represents the percentage of cells expressing these genes. (C) UMAP visualization of the tissue distribution in the myeloid compartment. (D) Heatmap showing the distribution of each myeloid cell population across different tissues. Cell numbers are normalized within each tissue and later calculated as proportions across tissues. Only tissues containing more than 50 myeloid cells in at least two donors were included. Asterisks mark significant enrichment in a given tissue relative to the remaining tissues (poisson regression stratified by donors, p < 0.05 after Benjamini-Hochberg (BH) correction). (E) Violin plot for genes differentially expressed in migratory dendritic cells across tissues. Color represents maximum-normalized mean expression of cells expressing marker genes. (F) smFISH visualisation of ITGAX, CCR7 and AIRE transcripts, validating the AIRE+ migratory dendritic cells in lung-draining lymph nodes.