Skip to main content
. Author manuscript; available in PMC: 2022 May 19.
Published in final edited form as: Nature. 2019 Jul 17;571(7766):580–583. doi: 10.1038/s41586-019-1391-0

Extended Data Figure 1. Reconstitution of TmrAB in lipid nanodiscs.

Extended Data Figure 1

a, SEC and SDS-PAGE (Coomassie) illustrating reconstitution of TmrAB in MSP1D1 nanodiscs. Column: Superdex 200 Increase 3.2/300. b, Isolation of TmrAB-MSP-Nb-complex for cryo-EM. Column: Shodex KW404-4F. c, Time course of [γ-32P]ATP hydrolysis of detergent-solubilized and nanodisc-reconstituted TmrABWT and TmrAEQB. d, ATPase activity of detergent-solubilized and nanodisc-embedded TmrAB (background corrected). TmrAEQB displays a drastically decreased ATPase activity in comparison to TmrABWT. The mean ± SD is calculated based on three different data points (time points in Figure 1c).