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. Author manuscript; available in PMC: 2022 Jun 20.
Published in final edited form as: Science. 2022 Apr 14;376(6595):844–852. doi: 10.1126/science.abn3810

Figure 3. The extranucleosomal linker DNA is gripped by the CENP-LN channel.

Figure 3

(A) Cartoon representation of CCANΔT-CENP-ANuc with cryo-EM density shown for extranucleosomal linker DNA (from CCANΔT-DNA cryo-EM density map). (B) The CENP-LN module features a positively-charged channel that complements the DNA duplex. Electrostatic surface charge shown for CENP-LN. (C and D). Details of the CENP-LN channel-DNA interaction. R169 of CENP-N inserts into the DNA minor groove. CENP-LN surface charge shown in C. (E) Cartoon schematic of the CCANΔT modules showing DNA path. (F and G) CENP-C binds to two sites on CCAN. The DEFxIDE motif binds to CENP-LN (E), whereas the FxxLFL motif binds to CENP-HIKM (G).