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. Author manuscript; available in PMC: 2022 Jun 20.
Published in final edited form as: Science. 2022 Apr 14;376(6595):844–852. doi: 10.1126/science.abn3810

Figure 4. Architecture of the full CCANΔC-DNA complex.

Figure 4

(A) Cryo-EM density map, and (B) molecular model of the CCANΔC-DNA complex with DNA map density shown in yellow. (C) CCAN assembles an enclosed chamber that topologically entraps linker DNA. (D) Electrostatic representation of the DNA-binding chamber formed by CENP-LN, CENP-TW and CENP-HIKHead modules shows a positively charged chamber that tightly grips DNA. (E) Schematic of CCAN showing DNA path through the CENP-LN, CENP-HIKM and CENP-TWSX modules. (F) The CENP-TWSX module resembles the canonical H3/H4 nucleosome tetramer, and partially wraps DNA.