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. Author manuscript; available in PMC: 2022 Nov 30.
Published in final edited form as: Nat Microbiol. 2022 May 30;7(6):856–867. doi: 10.1038/s41564-022-01132-w

Fig. 5. Producer and target range of the Nocardioides lysis-associated peptidase NlaP.

Fig. 5

a) Genomic region of Leaf245 (query) and exemplary strains containing similar gene neighborhoods compared to ASF05_00205 (nlaP, red) identified by IMG bidirectional best hit. The position of nlaP and nlaR are indicated in the genome map b) In vitro activity of candidate strains encoding nlaP and nlaR homologs and control Aeromicrobium strains not encoding a nlaP homolog. c) In vitro inhibition of a panel of At-SPHERE Actinobacteria by Aeromicrobium and Terrabacter strains (OD600= 1), the corresponding cell free supernatants (OD600 of suspended cells = 5) or by purified proteins (0.1 mg mL-1) indicated on the left side. Target strains are ordered by phylogeny (top) and were all normalized to an OD600 of 0.01 within the agar. Filled boxes indicate inhibition of the strain in the given condition, dark shade: complete clearance (1), light shade: partial clearance (2) as illustrated by the examples on the top right of the panel.