Table 3.

Degradation of 5′-ppp-mRNA to 5′-p-mRNA.

Compound	Cstock	Volume [µL]	Cfinal
ddH2O		ad 20 µL
5′-polyphosphatase reaction buffer	10×	2	1×
mRNA			max 5 µg
5′-polyphosphatase (Lucigen)	20 U/µL	1	1 U/µL