Table 9.
Mass spectrometer settings used for nucleosides and caps analyzed herein (CAV: cell acceleration voltage). Settings for quantifiers (quant.) and qualifiers (qual.) are shown.
| Compound (quantifier/qualifier) | Mode | Precursor ion (m/z) | Product ion (m/z) | Fragmentor voltage(V) | CAV (V) | Collision energy (V) | Retention time (min) |
|---|---|---|---|---|---|---|---|
| Am (qual.) | pos. | 282.1 | 69.1 | 109 | 9 | 25 | 4.2 |
| Am (quant.) | pos. | 282.1 | 136.1 | 109 | 9 | 9 | 4.2 |
| m6Am (qual.) | pos. | 296.1 | 108.0 | 121 | 9 | 68 | 4.6 |
| m6Am (quant.) | pos. | 296.1 | 150.0 | 121 | 9 | 8 | 4.6 |
| N6pAm (qual.) | pos. | 320.1 | 68.8 | 121 | 9 | 25 | 5.0 |
| N6pAm (quant.) | pos. | 320.1 | 174.0 | 121 | 9 | 9 | 5.0 |
| GpppG (qual.) | pos. | 789.1 | 134.7 | 150 | 9 | 121 | 3.0 |
| GpppG (quant.) | pos. | 789.1 | 151.8 | 150 | 9 | 57 | 3.0 |
| GpppG (qual.) | pos. | 789.1 | 248.4 | 150 | 9 | 21 | 3.0 |
| m7GpppG (qual.) | pos. | 803.1 | 151.7 | 135 | 9 | 61 | 4.3 |
| m7GpppG (qual.) | pos. | 803.1 | 165.7 | 135 | 9 | 53 | 4.3 |
| m7GpppG (quant.) | pos. | 803.1 | 248.1 | 150 | 9 | 21 | 4.3 |