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. Author manuscript; available in PMC: 2022 Dec 3.
Published in final edited form as: J Proteome Res. 2021 Nov 4;20(12):5402–5411. doi: 10.1021/acs.jproteome.1c00695

Figure 2. Influence of selected sample preparation parameters on the number of peptides identified from FFPE material.

Figure 2

(A) Ionic strength of the extraction buffer, (B) pH of the extraction buffer and (C) thickness of tissue slices. For each analysis, 50 ng of peptide digest were analysed by a 60 min nLC-MS/MS run. Error bars indicate the standard error of mean (SEM). For numbers of replicates see Supplemental Table S1.