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. Author manuscript; available in PMC: 2022 Jun 12.
Published in final edited form as: Nature. 2021 Oct 13;599(7883):120–124. doi: 10.1038/s41586-021-03986-2

Extended Data Figure 5. Selective killing of macrolides and tetracyclines.

Extended Data Figure 5

a. Time-kill curves. The survival of 12 abundant gut microbes was assessed over a 5 hour-treatment with either erythromycin, azithromycin or doxycycline. The graph shows the mean±SD of 3 independent experiments.

b. Live/dead staining of macrolide or tetracycline-treated E. coli ED1a and B. vulgatus. The left panel shows an overlay of phase contrast and fluorescence microscopy images of propidium iodide (PI)-stained E. coli ED1a or B. vulgatus before and 5 hours after erythromycin, azithromycin or doxycycline treatment. Cultures were concentrated before imaging; the scale bar is 10 μm. The right panel shows the corresponding quantification of live/dead-stained cells by flow cytometry with Sy to 9 on the x-axis (live cells) and PI on the y-axis (dead cells). As E. coli ED1a cells stain poorly with Sy to 9, we only quantified PI stained cells in this case. Both the total number of measured events (n) and the percentage of cells found in each region of the graph are indicated.