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. Author manuscript; available in PMC: 2022 Jun 18.
Published in final edited form as: Trends Biotechnol. 2021 Feb 8;39(10):1093–1109. doi: 10.1016/j.tibtech.2021.01.006

Table 3. Overview of Advantages and Disadvantages for Optical Measurement Principles Based on Species-Independent Parameters to Identify a Suitable Techniquea.

Absorbance Scattered light Autofluorescence
High cell numbers – + +
Different cell size – + –
Different cell walls – + +
Pigments + – +
Fluorescent metabolites – – +
a

+ indicates a preferred principle.