TABLE 3.
Experimental parameters critical to the unambiguous description of ligand bias
| Parameter | Pathway 1 | Pathway 2 | Pathway 3 |
|---|---|---|---|
| Transducer (‘pathway’) | β‐Arrestin 2 | Gi | Gq |
| Cell line | CHO | CHO | CHO |
| Receptor expression levels (fmol·mg−1 protein) | 300 | 300 | 300 |
| Time point for data collection (min) (for time‐resolved, a span) | 15–60 | 30 | 30 |
| Temperature (°C) | 20 | 20 | 20 |
| Reference ligand for bias | CP55,940 | CP55,940 | CP55,940 |
| Reference ligand for Emax | CP55,940 | CP55,940 | CP55,940 |
| Measured process | Recruitment | Accumulation | Accumulation |
| Measured molecule 1 a | CB1 receptor | cAMP | Inositol 1‐phosphate |
| Measured molecule 2 a (if any) | β‐Arrestin 2 | ‐ | ‐ |
| Signal detection technique | FRET | HTRF | HTRF |
Note: Table entries are examples only.
a
A measured molecule is a GPCR, transducer, effector or second messenger.