Table 1. Transgenic mouse models with increased mitochondrial function with concomitant loss of stemness in hematopoietic system.

		Phenotype
Model	Lethality	In vitro	In vivo	Reference
Mx-Cre+;FoxO1/3/4l/l	–	Defective HSC and CLP colony forming activity	Expansion of myeloid lineage and decrease in lymphoid commitment Loss of stem cell population in bone marrow Increased ROS levels in HSCs	47
FoxO3a–/–	–	Mild reduction in HSC clonogenic capacity	Deteriorated resistance to myelotoxic stress Increased HSC cycling	46
Atm–/–	By 24 weeks, post BM failure	Increase in intracellular H2O2 in LSK	Increased ROS accumulation BM failure as early as 24 weeks	43
Mx1-Cre-;Pdk2,4–/–	–	Decrease in the number of LSK cells in vitro Reduction in LT-HSC fraction in vitro	Loss of quiescence in HSCs and steady state hematopoiesis affected Accumulated mitochondrial ROS and diminished repopulation capacity Multilineage engraftment capacity remained unaffected	5
Mx-1-Cre;Tscfl/fl	–	Increase in the size and number of erythroid, macrophage, megakaryocyte and granulocyte colonies Increase in percentage of apoptotic cells in the aforesaid colonies	Increased mitochondrial biogenesis, increased mitochondrial DNA expression, increased ROS Active cycling of HSCs and loss of transplantation potential Premature aging of the hematopoietic system	52
Sirt7–/–	–	Not mentioned	Increased mitochondrial mass and ATP levels in HSC, increased mitochondrial function Loss of hematopoietic function	50
Vav1-Cre;Mtch2fl/fl	–	Decreased CFU-C potential in HSCs CFU-Cs from cKO mice less sensitive to radiation damage	Increased mitochondrial pathways in HSCs, increase in size and activity of mitochondria Increased HSC entry into cell cycle, decreased frequency of primitive HSCs	6
Mx1-Cre;HIF1afl/fl	–	–	Reduced HSC frequency and number with age	4
Germline Rev1–/–	Lifespan shorter than WT littermates	Rev1 HSC clonogenicity reduced	Loss of engraftment potential of HSCs Functional loss of HSCs starting as early as E14 FL	79
Rev1–/– Xpc–/–	≈3.5 months	–	Embryos born in sub-Mendelian ratios Smaller embryos than WT or single deficient littermates Major hematopoietic defects; very less HSC numbers starting as early as E14 FL Increased HSC cycling	79