Extended Data Figure. 4. SWI/SNF depletion potentiates YAP-induced reprogramming of neurons into NSCs.

a, Efficiency of Brm and Arid1a downregulation in neurons transduced with the indicated shRNA-encoding vectors, as measured by qRT-PCR (mean + s.d. of n=3 biologically independent samples). Shown is a representative experiment repeated twice with similar results.

b-c, Compendium of Fig. 2c. Neurons were infected with doxycycline-inducible YAP-encoding vectors or empty vector and the indicated shRNAs-encoding lentiviral vectors. Shown are representative images (b; scale bar, 300 μm) of the cultures after 14 days in NSC medium with doxycycline and quantifications of emerging (P0) neurospheres (c; mean + s.e.m. of four independent experiments; asterisk is shCo vs. shBrm (P value = 0.03) or shArid1A with YAPwt (P value = 0.03)).

d-e, Effect of ARID1A depletion on YAP-induced reprogramming of neurons. d, Syn1-Cre drives Arid1a knockout specifically in neurons as shown by genotyping. Neuron genomic DNA was compared to tail genomic DNA of the same Syn1-Cre; Arid1a^fl/+ mouse. Panels represent PCR bands for the indicated alleles. e, Control (Arid1a^+/+) and Arid1a^+/- (from Syn1-Cre; Arid1a^fl/+ mice) neurons were infected with inducible YAP-encoding vectors. Shown are representative images (left; scale bars, 300 μm), and the quantification (right; mean + s.e.m. of four independent experiments) of P0-neurospheres emerging from these cultures after doxycycline treatment in NSC medium. YAPS94A serves as negative control. e complements Fig. 2c and Extended Data Fig. 4b-c showing comparable results between shRNA and genetic attenuation of ARID1a.

P values were determined by unpaired two-sided t-test for a, and by two-sided Mann Whitney U test for c and e.