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. Author manuscript; available in PMC: 2022 Jul 5.
Published in final edited form as: ACS Chem Biol. 2019 Jun 27;14(7):1628–1636. doi: 10.1021/acschembio.9b00354

Figure 2. TG-FLIM imaging of protein aggregation in Q40 and α-syn strains across the lifespan of the animals.

Figure 2

(a) Fluorescence lifetimes at days 0, 3, 6, 10, 12, and 14 of adulthood for Q40 and α-syn compared to YFP controls. Squares and circles represent the average lifetime of the fluorescence from the head region of each imaged worm. Statistical analysis was performed using a one-way ANOVA, ***p < 0.005, ****p < 0.0001. The data shown are pooled from two biological replicates totalling ca. 40 worms per strain and time point. An F-test of YFP control lifetimes comparing data over the 15 days showed no significant deviation from a zero-slope, indicating that our data are compatible with a constant lifetime for the YFP control over the lifespan of the animal (p = 0.90). (b) Representative worm FLIM images for each day and each strain.