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. Author manuscript; available in PMC: 2022 Jul 5.
Published in final edited form as: Nat Comput Sci. 2021 Nov 22;1(11):732–743. doi: 10.1038/s43588-021-00155-3

Figure 6. Predicting LLPS propensities of other proteins and multiphasic compartmentalisation.

Figure 6

a Temperature–density phase diagrams for FUS PLD, LAF-1 RGG (WT) and two other variants of LAF-1 RGG for the Mpipi model. Filled symbols represent simulation data, while empty symbols depict estimated simulation critical points (see Methods). The horizontal dashed lines represent estimated Tθ (temperature of the coil-to-globule transition) for FUS PLD (magenta) and LAF-1 RGG (WT) (black) obtained with the Absinth potential. b, c Same as in a, but for four DDX4 variants and full FUS variants, respectively. d We simulate a mixture of PolyK (50 residues; 128 chains), PolyR (50 residues; 128 chains) and RNA (10 residues; 1280 chains) with an extended Mpipi model (see Methods and SI Fig. S4). The density profile along the simulation box’s long axis (L; normalised) is given for each mixture component. A simulation snapshot is provided below the density plot. The colour code in the snapshot is consistent with that used in the density plot. The mixture is simulated at T/Tc ≈ 0.8, where Tc is the critical temperature for liquid-vapour phase separation.