| Step | # cycles | Temperature | Time |
|---|---|---|---|
| 1 | 1 | 95°C | 2 min |
| 2 | 6 | 95°C | 30 sec |
| 60°C | 30 sec | ||
| 72°C | 30 sec | ||
| 3 | 1 | 72°C | 7 min |
| 4 | 1 | 4°C | Hold |
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