| Step | # cycles | Time | Temperature |
|---|---|---|---|
| Initial denaturation | 1 | 30 sec | 98°C |
| Denaturation | 3 | 10 sec | 98°C |
| Annealing/extension | 75 sec | 65°C | |
| Final extension | 1 | 5 min | 65°C |
| Hold | Indefinite | 4°C |
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