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. Author manuscript; available in PMC: 2022 Jul 20.
Published in final edited form as: Brain Behav Immun. 2020 Jan 30;87:473–488. doi: 10.1016/j.bbi.2020.01.018

Figure 5. Alpha-synuclein and Caspase-1.

Figure 5

(A), (B) - Fluorescence microscope images of monocytes which have taken up fluorescent alpha-synuclein-HiLyte™ Fluor 488 at 90minutes. Hoechst staining identifies cell nuclei. (a) 20X; (b) 40X.

(C), (D) - Monocyte fluorescent alpha-synuclein uptake in standard medium and in autologous serum – (C) percentage alpha-synuclein positive monocytes; (D) total monocyte MFI ratio. (Medium- Patients=35, Controls=35; Serum- Patients=27, Controls=28) (Patients=red; Controls=yellow).

(E),(F),(G),(H) - Alpha-synuclein and caspase-1 in monocyte 24 hour culture supernatants without (E)(F) and with (G)(H) LPS, in all participants and within risk groups. (Patients = red, Controls= yellow).

(All participants-Patients=18, Controls=20; Higher Risk Patients=15, Controls=16; Lower Risk Patients=3, Controls=4) (Patients=red; Controls=yellow).

(I), (J) Graphs showing significant relationship between alpha-synuclein and caspase-1 in serum (I) and monocyte lysates (J).

(K) ICICLE-Cambridge cohort - Relationship between alpha-synuclein and caspase-1 in plasma samples collected at study baseline.