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. Author manuscript; available in PMC: 2022 Jul 8.
Published in final edited form as: Science. 2021 Jan 1;371(6524):57–64. doi: 10.1126/science.abc7151

Fig. 2. Disome formation is nascent chain dependent.

Fig. 2

A) DiSP was performed on lysates treated with increasing Proteinase K (PK, n = 1) concentrations or with Puromycin (Puro, n = 2) to degrade or release nascent chains. Both treatments resulted in a large depletion of genes with ≥ 2-fold higher footprint density in the disome compared to the monosome fraction.

B) Metagene enrichment profiles (disome / monosome) aligned to translation start of all detected genes in PK (top) and Puro (bottom) DiSP experiments.

C) Enrichment profiles (disome / monosome) of DCTN1 of untreated DiSP samples and samples treated with increasing concentrations of Proteinase K (PK, top) or with Puromycin (Puro, bottom).