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. Author manuscript; available in PMC: 2022 Jul 23.
Published in final edited form as: Am J Transplant. 2020 Sep 15;21(3):978–992. doi: 10.1111/ajt.16266

Figure 6.

Figure 6

IL-33-Tregs upregulate genes critical for suppression and migration of Tregs to tissue. CBA/Ca (H-2k) mice were injected with either PBS (control, black) or recombinant IL-33 (1 μg/d, red) for 6 consecutive days, and spleens were harvested 24 h after last injection. RNA from splenocyte-derived CD4+CD25+ Tregs were FACS-sorted from PBS- (n = 3) or IL-33-treated (n = 4) H-2k mice for gene expression analysis. (A) Volcano plot reveals the most differentially expressed genes, relative to a baseline of control mice. Genes associated with Treg suppressive function (B, blue) and genes associated with Treg homing (C, green) are represented in scatter violin plots. Adjusted P value calculated with control of Benjamini-Yekutieli False Discovery Rate (FDR) (adjusted P > .05 considered significant, FDR thresholds indicated within volcano plot) [Color figure can be viewed at wileyonlinelibrary.com]