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. Author manuscript; available in PMC: 2022 Jul 28.
Published in final edited form as: Nat Protoc. 2020 Jul 10;15(8):2568–2588. doi: 10.1038/s41596-020-0344-2

Fig. 4. Schematic of TMT experimental design.

Fig. 4

Different treatments (t) can be combined in the same TMT experiment. Total proteomes are obtained from the total cell lysates, TMT labeled and combined into a single sample. Different RBPomes are obtained independently, labeled and combined into a second sample.