Table 3. Feeding solution composition.

				Final requested feeding solution volume
Number	Reagent	stock conc.	final conc.	25 μL	50 μL	100 μL	200 μL	300 μL	400 μL
1	HEPES KOH pH=8	1 M	83.3 mM	2.08	4.17	8.33	16.67	25	33.33
2	Magnesium acetate	1 M	21.2 mM	0.53	1.06	2.12	4.24	6.36	8.48
3	Potassium acetate	1 M	75.5 mM	1.89	3.78	7.55	15.1	22.66	30.2
4	Ammonium acetate	5.2 M	236.4 mM	1.14	2.27	4.55	9.09	13.64	18.18
5	PEG - 6000	50%	4.54%	2.27	4.54	9.08	18.16	27.24	36.32
6	3-PGA	0.5 M	60.1 mM	3	6.01	12.01	24.02	36.04	48.04
7	Amino acids - mixture I	50 mM	3.8 mM	1.89	3.78	7.56	15.12	22.68	30.24
8	Amino acids - mixture II	50 mM	3.8 mM	1.89	3.78	7.56	15.12	22.68	30.24
9	ATP	100 mM	1.8 mM	0.45	0.91	1.81	3.62	5.43	7.24
10	GTP	50 mM	1.5 mM	0.76	1.51	3.02	6.04	9.06	12.08
11	UTP	100 mM	1.2 mM	0.3	0.61	1.21	2.42	3.63	4.84
12	IPTG	100 mM	1.5 mM	0.38	0.76	1.51	3.02	4.53	6.04
13	Glucose	2 M	200 mM	2.5	5	10	20	30	40
14	H2O UPW			5.92	11.83	23.7	47.38	71.06	94.76