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. Author manuscript; available in PMC: 2023 Jan 7.
Published in final edited form as: Nat Ecol Evol. 2022 Jul 7;6(8):1155–1164. doi: 10.1038/s41559-022-01797-w

Figure 2. L populations harbor fewer nonsynonymous and neo-functionalizing variants.

Figure 2

A. The average number of non-synonymous variants per GFP molecule is significantly lower in L (blue) than in H (red) populations (two-tailed unpaired t-test, n=6, p=0.003, Cohen’s d=2.95±1.87, t=5.12, 95%CI= 0.28, 0.86, df= 5Methods). B. The average number of neo-functionalizing variants per GFP molecule is significantly lower in L populations (blue) than in H (red) populations for six generations of directed evolution (two-tailed unpaired t-test, n=6, p=0.007, Cohen’s d=2.52±1.73, t=4.37, 95%CI= 0.096, 0.37, df= 5, Methods). Not all sequenced GFP molecules harbor the neo-functionalizing variant, which is why the average is lower than one in all experiments. In both panels each dashed line corresponds to data from one replicate population, and the solid line represents the mean of the four replicate populations.