| Step 1 | 98°C | 45 s |
| Step 2 | 98°C | 15 s |
| Step 3 | 60°C | 30 s |
| Step 4 | 72°C | 30 s |
| Step 5 | Go to Step 2 | |
| Step 6 | 72°C | 60 s |
| Step 7 | 4°C | Hold |
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