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. Author manuscript; available in PMC: 2022 Aug 13.
Published in final edited form as: Nat Protoc. 2022 Feb 4;17(2):445–475. doi: 10.1038/s41596-021-00651-w
Reagent Stock Conc. Volume Work Conc.
PCR Grade Water - 48.4 mL -
Tris pH8 1 M 500 μL 10 mM
NaCl 4 M 125 μL 10 mM
Igepal CA-630 10% vol/vol 1 mL 0.2% vol/vol
cOmplete Protease Inhibitor Cocktail - 1 tablet