Reagent | Stock Conc. | Volume | Work Conc. |
---|---|---|---|
PCR Grade Water | - | 48.4 mL | - |
Tris pH8 | 1 M | 500 μL | 10 mM |
NaCl | 4 M | 125 μL | 10 mM |
Igepal CA-630 | 10% vol/vol | 1 mL | 0.2% vol/vol |
cOmplete Protease Inhibitor Cocktail | - | 1 tablet | 1× |
Reagent | Stock Conc. | Volume | Work Conc. |
---|---|---|---|
PCR Grade Water | - | 48.4 mL | - |
Tris pH8 | 1 M | 500 μL | 10 mM |
NaCl | 4 M | 125 μL | 10 mM |
Igepal CA-630 | 10% vol/vol | 1 mL | 0.2% vol/vol |
cOmplete Protease Inhibitor Cocktail | - | 1 tablet | 1× |