Reagent	Digest	Control 1
DpnII digested 3C library
Nuclei in 1× DpnII buffer	200 μL	15 μL
PCR-grade water	434 μL	227.5 μL
10× DpnII buffer	60 μL	28.5 μL
20% vol/vol SDS (0.28% final conc.)	10 μL	4 μL
NlaIII digested 3C library
Nuclei in 1× CutSmart® buffer	200 μL	15 μL
PCR-grade water	393.5 μL	227.5 μL
10× CutSmart® buffer	60 μL	28.5 μL
20% vol/vol SDS (0.28% final conc.)	9.5 μL	4 μL