Fig. 1. Experimental Strategy.
Cryo-preserved African trypanosome isolates recovered from sleeping sickness patients were subjected to various group/species specific PCR detections to identify human infective Trypanosoma brucei rhodesiense. Pathogenicity/virulence studies of four selected T. b. rhodesiense isolates was undertaken in mouse. Consequently, the establishment of most and least virulent isolates in tsetse fly was undertaken. Tbr: Trypanosoma brucei rhodesiense; Tbg: Trypanosoma brucei gambiense; and Te: Trypanosoma evansi.
