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. Author manuscript; available in PMC: 2022 Aug 22.
Published in final edited form as: Nature. 2021 Nov 10;599(7885):497–502. doi: 10.1038/s41586-021-04030-z

Extended Data Fig. 2. ShCas12k cryo-EM image processing workflow and model validation.

Extended Data Fig. 2

a, Cryo-EM image processing workflow for the ShCas12k-sgRNA-target DNA complex. Fourier Shell Correlations (FSC) of ShCas12k reconstruction from two independently refined half-maps. The gold-standard cutoff (FSC = 0.143) is marked with a blue line. b, Final electron density map colored according to the local resolution. c, Validation of Cas12k-sgRNA-target DNA structure model.