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. Author manuscript; available in PMC: 2022 Aug 26.
Published in final edited form as: Gut. 2019 May 10;69(2):380–392. doi: 10.1136/gutjnl-2018-317423

Figure 6. Silencing of OGT increases the size of HCVcc.

Figure 6

(A) Representative pictures of HCV particles generated in Huh7.5.1 cells transfected with non-targeting siRNA (siCtrl) or siOGT. (B-F) Comparative analysis of particle size distribution for immunocapture (IC) from HCV particles produced in Huh7.5.1 cells transfected with siCtrl or siOGT prior to infection with HCVcc (JcR2a) following sucrose-cushion purification (B) or iodixanol gradient fractionation (C-F) of HCVcc. HCVcc were transferred via anti-E2 antibody AR3A on electron microscopy (EM) grids through IC. Particle size distribution was assessed from a series of randomly acquired electron micrographs with Image-J software (NIH). Results from one of three (A-B) or two (C-F) independent experiments are shown. Black lines: size distribution of immunocaptured HCVcc produced in siCtrl-transfected cells. Grey lines: size distribution of immunocaptured HCVcc produced in siOGT-transfected cells.