REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Sheep anti-CGRP (1:400)	Enzo Life	Cat# BML-CA1137; RRID: AB_2050885
Rabbit anti-CGRP (1:1000)	Peninsula Laboratories	Cat# T-4032; RRID: AB_2313775
Isolectin B4 (IB4), conjugated to biotin (1:100)	Sigma-Aldrich	Cat# L2140; RRID: AB_2313663
Mouse anti-NF200 (1:250)	Sigma-Aldrich	Cat# N0142; RRID: AB_477257
Rabbit anti-NCX3 (1:1000)	Swant	Cat# 95209
Chicken anti-NeuN (1:500)	Millipore	Cat# ABN91; RRID: AB_11212808
Sheep anti-Tyrosine Hydroxylase (1:200)	Millipore	Cat# AB1542; RRID: AB_90755
Chicken anti-GFP (1:1000)	Abcam	Cat# ab13970; RRID: AB_300798
GAPDH (1:1000)	Abcam	Cat# ab181602
Donkey anti-rabbit IgG Alexa 488 (1:1000)	Thermo Fisher Scientific	Cat# A-21206; RRID: AB_2535792
Streptavidin Pacific Blue (1:500)	Thermo Fisher Scientific	Cat# S11222
Donkey anti-mouse IgG Alexa 488 (1:1000)	Thermo Fisher Scientific	Cat# A-21202; RRID: AB_141607
Goat anti-mouse IgG Pacific blue (1:500)	Thermo Fisher Scientific	Cat# P31582; RRID: AB_10374586
Donkey anti-sheep IgG Alexa 488 (1:1000)	Thermo Fisher Scientific	Cat# A-11015; RRID: AB_2534082
Goat anti-chicken Alexa 488 (1:1000)	Thermo Fisher Scientific	Cat# 11039; RRID: AB_2534096
Anti-rabbit HRP-conjugated (1:5000)	Fisher Scientific	Cat# NA934VS
Anti-mouse HRP-conjugated (1:10000)	Fisher Scientific	Cat# NA931VS
Bacterial and virus strains
ssAAV-1/2-shortCAG-mSlc8a3_myc_FLAG-WPRE-SV40p(A) (capsid 1)	VVF - custom	This paper
ssAAV-1/2-shortCAG-EGFP-WPRE-SV40p(A) (capsid 1)	VVF - repository	Cat# v587-1
Chemicals, peptides, and recombinant proteins
Formaldehyde solution	Sigma-Aldrich	Cat# 252549
Ionomycin	Sigma-Aldrich	Cat# I0634-1MG
Critical commercial assays
RNAscope 2.5 HD Reagent Kit-RED	Biotechne	Cat# 322350
RNAscope® Fluorescent Multiplex Detection Reagent kit	Biotechne	Cat# 320851
LightCycler 480 SYBR Green Master mix	Roche	Cat# 04707516001
In Fusion® Cloning kit	Takara Bio	Cat# 638909
Jet PEI DNA Transfection Reagent	Polyplus Transfection	Cat# 101-10N
Deposited data
Raw data showing Ca2+ imaging selection protocol and decay kinetics analysis	https://github.com/Teodora-trend/calcium_imaging	https://doi.org/10.5281/zenodo.6529629
Experimental models: Cell lines
HEK-293	N/A	N/A
Experimental models: Organisms/strains
Slc8a3tm1Sso mouse line	Sokolow et al., 2004	N/A
Gad67-EGFP mouse line	Tamamaki et al., 2003	Tissue from Professor H. Zeilhofer
GlyT2-eGFP mouse line	Zeilhofer et al., 2005	Tissue from Professor H. Zeilhofer
vGluT2-eGFP mouse line	Gong et al., 2003	Tissue from Professor H. Zeilhofer
Phox2aCre x Rosa26LSL-tdTomato		Tissue from Professor A. Todd
C57Bl/6J mouse line used for spinal injections	Charles River	Strain code 632
Oligonucleotides
NCX3_F CAGATAAGCGACTGCTCTTC	Thermo Fisher Scientific	Sokolow et al., 2004
NCX3_R CCTGGCTTCAGAACCACAGTG	Thermo Fisher Scientific	Sokolow et al., 2004
NCX3_Neo TCGACTAGAGGATCAGCTT	Thermo Fisher Scientific	Sokolow et al., 2004
NCX3-AC_F GGGCCCCCGCATGGTGGATA	Thermo Fisher Scientific	Michel et al., 2014
NCX3-AC_R CAGCTTCCTGTCTGTCACTTCTGGA	Thermo Fisher Scientific	Michel et al., 2014
NCX3-B_F GCATATGGGGAGCTGGAGT	Thermo Fisher Scientific	Michel et al., 2014
NCX3-B_R GTTCACCAAGGGCAATGAAG.	Thermo Fisher Scientific	Michel et al., 2014
Cloning Oligo 1 (BamHI) (Insert 1) Forward GCAAAGAATTGGATCCGCCACCATG GCGTGGTTA	Thermo Fisher Scientific	This paper
Cloning Oligo 2 (Insert 1) (EcoRI) Reverse GCTTGATATCGAATTCTTAAACCTTATC GTCGTCATCCTTG	Thermo Fisher Scientific	This paper
RNAscope® Probe- Mm-Slc8a3	Biotechne	Cat# 523681
RNAscope® Probe- Mm-Lypd1	Biotechne	Cat# 318361-C2
RNAscope® Probe- Mm-tdTomato	Biotechne	Cat# 317041-C2
Recombinant DNA
pAAV CAG-GFP	From Edward Boyden (Addgene plasmid # 37825; http://n2t.net/addgene:37825)	RRID: Addgene_37825
Slc8a3-B (Myc-DDK-tagged)	Origene	Cat# MR211189
Software and algorithms
Plink software for genetic data analysis and GWAS	Chang et al., 2015	https://www.cog-genomics.org/plink/1.9/
R statistical software		https://cran.r-project.org/
FastMan package for R for manhattan plot visualization	Paria et al., 2022	https://github.com/kaustubhad/fastman
R package for GWAS power calculation	Schmid et al., 2019	https://github.com/kaustubhad/gwas-power
SHAPEIT2 software for genetic data phasing	O’Connell et al., 2014	https://mathgen.stats.ox.ac.uk/genetics_software/shapeit/shapeit.html
IMPUTE2 software for genotype imputation	Howie et al., 2012	https://mathgen.stats.ox.ac.uk/impute/impute_v2.html
ADMIXTURE software for genetic ancestry estimation	Alexander et al., 2009	http://dalexander.github.io/admixture/
GCTA software for gene-level association GCTA-fastBATtest	Bakshi et al., 2016	https://cnsgenomics.com/software/gcta/#Gene-basedtest
Illumina GenomeStudio software for genotype calling		https://www.illumina.com/techniques/microarrays/array-data-analysis-experimental-design/genomestudio.html
GraphPad Prism 9	GraphPad	https://www.graphpad.com/
ImageJ	National Institutes of Health	https://imagej.nih.gov/ij/
FIJI software for pixel-based color quantification	FIJI	https://imagej.net/Fiji
Avidemux	Avidemux	https://avidemux.en.softonic.com/mac
Microsoft Excel	Microsoft	https://www.microsoft.com/en-gb/
Matlab	Mathworks	https://uk.mathworks.com/products/matlab.html
BioRender		https://biorender.com
Neurolucida	MBF Bioscience	https://www.mbfbioscience.com/neurolucida-version-2020
Other
GWAS summary statistics from the CANDELA cohort	GWAS Central	https://www.gwascentral.org/study/HGVST3308
GTEx expression data for SLC8A3	GTEx	https://www.gtexportal.org/home/gene/SLC8A3
GTEx expression data for S100A16	GTEx	https://www.gtexportal.org/home/gene/S100A16
ENCODE expression data for SLC8A3	ENCODE	https://screen.wenglab.org/geApp/?assembly=GRCh38&gene=SLC8A3