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. Author manuscript; available in PMC: 2022 Sep 22.
Published in final edited form as: J Struct Biol. 2022 Jun 2;214(3):107872. doi: 10.1016/j.jsb.2022.107872

Fig. 10.

Fig. 10

Graphical walkthrough over directional picking results: imported tomogram displayed with IMOD-3dmod [a], denoised tomogram with the plugin scipion-em-tomo3d and displayed with IMOD-3dmod [b], membrane segmentation with the plugin scipion-em-tomosegmemtv, displayed with IMOD-3dmod [c] and manual annotation of the segmented membranes, carried out and displayed with the Membrane Annotator tool from the plugin scipion-em-tomosegmemtv [d]. Sub-figures [e] to [h] show the results of the preseg, graphs, filaments, and picking, respectively, steps of the directional picking procedure with PySeg. Preseg results in [e] are displayed with xmipp-Dataviewer and the rest, for the membrane labeled as 3, with viewer tomoviz from the plugin scipion-em-tomoviz. Sub-figure [i] represents the best 2D class obtained with the 2D classification from the plugin scipion-em-pyseg, represented as a rotational average around the green axis (which is why the class representative is half of the particle box). Sub-figure [j] shows a volume resulting from averaging picked particles after some STA steps (carried out with the plugin scipion-em-reliontomo, Relion 3 version) in ChimeraX viewer (contained in the plugin scipion-em-chimera).