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. Author manuscript; available in PMC: 2022 Sep 22.
Published in final edited form as: J Struct Biol. 2022 Jun 2;214(3):107872. doi: 10.1016/j.jsb.2022.107872

Fig. 2.

Fig. 2

Schematic representation of a “classical” cryoET workflow. Partial tilt series movies (multi-frame images) showing only 3 different tilt angle acquisitions [a]. A beam-induced motion correction method should align all frames to get tilt series images [b]. A hypothetical 19 angles tilt series representation is presented in [c]. Tilt series alignment methods will estimate the transformation matrices to align the whole tilt series and render an aligned tilt series [d]. Tomogram reconstruction methods will generate a tomogram [e]. Tomogram segmentation and picking methods will help to identify 3D coordinates of interest [e - red circles]. Tomogram extraction methods will extract the 3D boxes (subtomograms) containing the particles of interest [f]. STA methods will calculate a final 3D average [g].