Figure 5. Neutralization of G-CSF abrogates the augmented monocytes, bone marrow progenitors and T_H2 cytokines observed in neutrophil depleted HDM exposed mice.

(A) Balb/c mice were administered HDM intranasally (i.n.) 3 times per week for 1 week. At 24 hours prior to each HDM administration, mice were treated with either 100 μg of neutrophil depleting antibody, 1A8, or isotype control antibody, 2A3. To neutralize G-CSF, mice were also administered 100 μg anti-G-CSF or isotype control, 2A3, i.p. at 24 hours prior to each HDM dose. At 24 hours post final HDM administration, BAL, lung tissue and bone marrow were collected (Ŧ). The number of lung and airway neutrophils (B) and lung Ly6C^low and Ly6C^high monocytes (C) were determined by flow cytometry. (D) The percentage of MDPs and CDPs within the bone marrow were assessed by flow cytometry. (E) The concentrations of BALF IL-4, IL-5 and IL-13 were assessed by ELISA. (F) Geometric mean of IL-13 by lung ILC2s was assessed by flow cytometry. Figures present data combined from 2 independent experiments with 4-6 mice per group in each experiment. Results depicted as mean ± SEM. *P<0.05, **P<0.01, ***P<0.001 using Mann–Whitney statistical test.