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. Author manuscript; available in PMC: 2022 Sep 22.
Published in final edited form as: Sci Immunol. 2019 Nov 8;4(41):eaax7006. doi: 10.1126/sciimmunol.aax7006

Figure 6. Co-administration of recombinant G-CSF augments numbers of HDM-induced neutrophils, monocytes and bone marrow progenitors and ILC2 cytokine responses.

Figure 6

(A) Balb/c mice were administered PBS or HDM intranasally (i.n.) 3 times per week for 1 week. Mice were also administered 100 ng recombinant G-CSF i.n. (in 50 μl PBS) and 2 μg recombinant G-CSF i.p. (in 2000 μl PBS), or respective PBS controls, daily throughout the study. At 24 hours post final HDM administration, BAL, lung tissue and bone marrow were collected (Ŧ). The number of lung and airway neutrophils (B) and lung Ly6Clow and Ly6Chigh monocytes (C) were determined by flow cytometry. (D) The percentage of MDPs and CDPs within the bone marrow were assessed by flow cytometry. (E) The concentrations of BALF IL-4, IL-5 and IL-13 were assessed by ELISA. (F) Geometric mean of IL-13 by BAL ILC2s was assessed by flow cytometry. Figures present data from 1 experiment with 4-6 mice per group in each experiment. Results depicted as mean ± SEM. *P<0.05, **P<0.01 using Mann– Whitney statistical test.