Figure 7. G-CSF augments T_H2 cytokine production from IL-33 expanded airway Csf3r-expressing ILC2s.

Balb/c mice were administered HDM intranasally (i.n.) 3 times per week for 1 week. At 24 hours post final HDM administration, BAL was collected. (A) Relative expression of mRNA Csf3r in T cells and ILC2s isolated by FACS from the BAL of HDM exposed mice, as determined by qPCR. (B) Balb/c mice were administered 1 μg recombinant IL-33 i.n. 3 times per week for 1 week. At 24 hours post final IL-33 administration, BAL was collected. (C) Relative expression of mRNA Csf3r in T cells and ILC2s isolated by FACS from the BAL of IL-33 exposed mice, as determined by qPCR. In some experiments, 100 ng recombinant G-CSF was co-administered with IL-33 (B), and CD4⁺ T cells and ILC2s were isolated from the airways by FACS, at 24 hours post final IL-33 administration, for subsequent mRNA gene expression analysis. (D) Relative expression of IL-4, and IL-13 in T cells and ILC2s derived from BAL, as determined by qPCR. (E) From the same experiments, BALF concentrations of IL-5 and IL-13 were determined by ELISA. Figures present data from 1 experiment with 4-6 mice per group in each experiment. Results depicted as mean ± SEM. *P<0.05, **P<0.01 using Mann–Whitney statistical test.